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Activin A Receptor Type IC Antibody, Cy5.5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-5777R-Cy5.5-100uL
Product Name Activin A Receptor Type IC Antibody, Cy5.5 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ACTRIC/Activin A Receptor Type IC
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 130399
Swiss Prot Q8NER5
Subcellular location Cell membrane
Synonyms ALK7; ACVRLK7; Activin receptor type-1C; Activin receptor type IC; ACTR-IC; Activin receptor-like kinase 7; ALK-7; ACVR1C
Background Serine/threonine protein kinase which forms a receptor complex on ligand binding. The receptor complex consisting of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators, SMAD2 and SMAD3. Receptor for activin AB, activin B and NODAL. Plays a role in cell differentiation, growth arrest and apoptosis.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Activin A Receptor Type IC Antibody, Cy5.5 Conjugated (BS-5777R-CY5-5) validated for, and at what dilution?

BS-5777R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Activin A Receptor Type IC Antibody, Cy5.5 Conjugated (BS-5777R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Rabbit.

How should BS-5777R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Activin A Receptor Type IC Antibody, Cy5.5 Conjugated (BS-5777R-CY5-5)?

KLH conjugated synthetic peptide derived from human ACTRIC/Activin A Receptor Type IC

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5777R-CY5-5)?

Cy5.5 on BS-5777R-CY5-5: excitation / emission 675nm/694nm.

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