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ADAM23 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-5853R-Cy5.5-100uL
Product Name ADAM23 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADAM23
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8745
Swiss Prot O75077
Subcellular location Secreted, Cell membrane
Synonyms MDC3; MDC-3; Disintegrin and metalloproteinase domain-containing protein 23; ADAM 23; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3; ADAM23
Background May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADAM23 Polyclonal Antibody, Cy5.5 Conjugated (BS-5853R-CY5-5) validated for, and at what dilution?

BS-5853R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAM23 Polyclonal Antibody, Cy5.5 Conjugated (BS-5853R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.

How should BS-5853R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADAM23 Polyclonal Antibody, Cy5.5 Conjugated (BS-5853R-CY5-5)?

KLH conjugated synthetic peptide derived from human ADAM23

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5853R-CY5-5)?

Cy5.5 on BS-5853R-CY5-5: excitation / emission 675nm/694nm.