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ADAM28 Polyclonal Antibody, APC-Cy5.5 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
Overview
Catalog # bs-5854R-APC-Cy5.5-100uL
Product Name ADAM28 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ADAM28
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10863
Swiss Prot Q9UKQ2
Subcellular location Secreted, Cell membrane
Synonyms MDCL; MDC-L; eMDCII; ADAM 28; eMDC II; Disintegrin and metalloproteinase domain-containing protein 28; Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; ADAM28; ADAM23
Background May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADAM28 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5854R-APC-CY5-5) validated for, and at what dilution?

BS-5854R-APC-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAM28 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5854R-APC-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.

How should BS-5854R-APC-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADAM28 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5854R-APC-CY5-5)?

KLH conjugated synthetic peptide derived from human ADAM28

What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-5854R-APC-CY5-5)?

APC-Cy5.5 on BS-5854R-APC-CY5-5: excitation / emission 650nm/694nm.

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