Calreticulin Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5913R-BF350-100uL |
| Product Name | Calreticulin Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Calreticulin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 811 |
| Swiss Prot | P27797 |
| Subcellular location | Cytoplasm, Secreted, Cell membrane, Extracellular matrix |
| Synonyms | RO; CRT; SSA; cC1qR; HEL-S-99n; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 6; ERp6; HACBP; grp6; CALR; CRTC |
| Background | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Calreticulin Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5913R-BF350) validated for, and at what dilution?
bs-5913R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calreticulin Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5913R-BF350) react with?
Tested reactivity: Human.
How should bs-5913R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Calreticulin Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-5913R-BF350)?
KLH conjugated synthetic peptide derived from human Calreticulin
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-5913R-BF350)?
AbBy Fluor-350 on bs-5913R-BF350: excitation / emission 346nm/442nm.