Annexin A9 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5916R-Cy3-100uL |
| Product Name | Annexin A9 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Annexin A9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8416 |
| Subcellular location | Cytoplasm, Secreted, Extracellular matrix |
| Synonyms | annexin 31; annexin A9; annexin XXXI; ANX31; ANXA9; ANXA 9; ANXA-9; pemphaxin; ANXA9_HUMAN . |
| Background | ANXA9 may act as a low affinity receptor for acetylcholine and is expressed in the stratified squamous skin epithelium, but not in epithelia of other types (at protein level). ANXA9 is one of the target molecules recognized by auto in patients with pemphigus vulgaris, a rare, autoimmune skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Annexin A9 Polyclonal Antibody, Cy3 Conjugated (bs-5916R-Cy3) validated for, and at what dilution?
bs-5916R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-5916R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Annexin A9 Polyclonal Antibody, Cy3 Conjugated (bs-5916R-Cy3)?
KLH conjugated synthetic peptide derived from human Annexin A9
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-5916R-Cy3)?
Cy3 on bs-5916R-Cy3: excitation / emission 512,550nm/570,615nm.