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YB1 Polyclonal Antibody, APC-Cy5.5 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Dog
  • Pig
  • Chicken
Overview
Catalog # bs-5921R-APC-Cy5.5-100uL
Product Name YB1 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat, Bovine, Dog, Pig, Chicken
Specifications
Conjugation APC-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human YBX-1/YB1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4904
Synonyms BP 8; CBF-A; YB1; CCAAT binding transcription factor I subunit A; YBX1; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; MDR NF1; NSEP 1; NSEP1; Nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; p50; Q15905; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB 1. YB-1; YBOX1_HUMAN; YBX 1; YBX1.
Background The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumour aggressiveness and belongs to the cold-shock domain family.
Application Dilution
WB 1:500-2000
IF FCM1:100

Frequently asked questions

Which applications is YB1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5921R-APC-CY5-5) validated for, and at what dilution?

BS-5921R-APC-CY5-5 is validated for WB (1:500-2000); FCM (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does YB1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5921R-APC-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken.

How should BS-5921R-APC-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise YB1 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-5921R-APC-CY5-5)?

KLH conjugated synthetic peptide derived from human YBX-1/YB1

What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-5921R-APC-CY5-5)?

APC-Cy5.5 on BS-5921R-APC-CY5-5: excitation / emission 650nm/694nm.

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