Apg10 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-5991R-Cy5.5-100uL |
| Product Name | Apg10 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Apg10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 83734 |
| Subcellular location | Cytoplasm |
| Synonyms | Apg 10; Apg-10; APG 10L; APG10 autophagy 10 like; APG10 like; APG10-like; APG10L; ATG 10; ATG10; ATG10 autophagy related 10 homolog S. cerevisiae; ATG10 autophagy related 10 homolog; ATG10_HUMAN; Autophagy related protein 10; Autophagy-related protein 10; Ubiquitin-like-conjugating enzyme ATG10 |
| Background | E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Apg10 Polyclonal Antibody, Cy5.5 Conjugated (BS-5991R-CY5-5) validated for, and at what dilution?
BS-5991R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-5991R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Apg10 Polyclonal Antibody, Cy5.5 Conjugated (BS-5991R-CY5-5)?
KLH conjugated synthetic peptide derived from human Apg10
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-5991R-CY5-5)?
Cy5.5 on BS-5991R-CY5-5: excitation / emission 675nm/694nm.