XAB2 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6080R-Cy3-100uL |
| Product Name | XAB2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human XAB2/HCNP. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56949 |
| Subcellular location | Nucleus |
| Synonyms | 0610041O14Rik; adapter protein ATH 55; Adapter protein ATH-55; Ath55; AV025587; Crn related protein kim1; crn-related protein kim1; DKFZp762C1015; HCNP; HCNP protein; HCRN; KIAA1177; NTC90; PP3898; Pre-mRNA-splicing factor SYF1; Protein HCNP; RNA splicing factor; SYF1; SYF1 homolog; SYF1 homolog, RNA splicing factor; SYF1_HUMAN; Xab2; XPA binding protein 2; XPA-binding protein 2. |
| Background | XAB2 protein (XPA binding protein 2) was first identified through its interaction with XPA, a key factor in nucleotide excision repair pathways. XAB2 therefore appears to be involved in transcription coupled DNA repair and transcription. Immunoprecipitation experiments have also demonstrated that a fraction of XAB2 interacts with the transcription coupled repair specific proteins CSA and CSB as well as with RNA polymerase II. Microinjection experiments with XAB2 resulted in the inhibition of both transcription coupled repair and transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is XAB2 Polyclonal Antibody, Cy3 Conjugated (bs-6080R-Cy3) validated for, and at what dilution?
bs-6080R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6080R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise XAB2 Polyclonal Antibody, Cy3 Conjugated (bs-6080R-Cy3)?
KLH conjugated synthetic peptide derived from human XAB2/HCNP.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6080R-Cy3)?
Cy3 on bs-6080R-Cy3: excitation / emission 512,550nm/570,615nm.