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WASF2 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Chicken
Overview
Catalog # bs-6243R-Cy5.5-100uL
Product Name WASF2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Sheep, Chicken
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human WASF2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10163
Subcellular location Cytoplasm, Cell membrane
Synonyms Protein WAVE-2; SCAR 2; SCAR; SCAR2; Verprolin homology domain-containing protein 2; WASF2; WASF2_HUMAN; WASP family protein member 2; WASP-family protein member 2; WAVE 2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Wiskott-Aldrich syndrome protein family verprolin-homologous protein; DICTYOSTELIUM; WASF2_HUMAN.
Background This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is WASF2 Polyclonal Antibody, Cy5.5 Conjugated (BS-6243R-CY5-5) validated for, and at what dilution?

BS-6243R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should BS-6243R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise WASF2 Polyclonal Antibody, Cy5.5 Conjugated (BS-6243R-CY5-5)?

KLH conjugated synthetic peptide derived from human WASF2

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6243R-CY5-5)?

Cy5.5 on BS-6243R-CY5-5: excitation / emission 675nm/694nm.

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