4 Hydroxynonenal Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6313R-BF647-100uL |
| Product Name | 4 Hydroxynonenal Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Others |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH-conjugated 4-hydroxynonenal |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | 4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; 4 HNE; 4HNE; 4-HNE; E-4-Hydroxynonenal-dimethylacetal, 4-HNE-DMA. |
| Background | Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress 4-HNE has been hypothesized to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is 4 Hydroxynonenal Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6313R-BF647) validated for, and at what dilution?
bs-6313R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6313R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 4 Hydroxynonenal Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-6313R-BF647)?
KLH-conjugated 4-hydroxynonenal
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-6313R-BF647)?
AbBy Fluor-647 on bs-6313R-BF647: excitation / emission 650nm/665nm.