WISP1 Polyclonal Antibody, Biotin Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6321R-Biotin-100uL |
| Product Name | WISP1 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WISP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 8840 |
| Swiss Prot | O95388 |
| Subcellular location | Secreted |
| Synonyms | CCN4; WISP1c; WISP1i; WISP1tc; WNT1-inducible-signaling pathway protein 1; WISP-1; CCN family member 4; Wnt-1-induced secreted protein; WISP1 |
| Background | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is WISP1 Polyclonal Antibody, Biotin Conjugated (bs-6321R-Biotin) validated for, and at what dilution?
bs-6321R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6321R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WISP1 Polyclonal Antibody, Biotin Conjugated (bs-6321R-Biotin)?
KLH conjugated synthetic peptide derived from human WISP1