WISP1 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-6321R-Cy5-100uL |
| Product Name | WISP1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WISP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8840 |
| Swiss Prot | O95388 |
| Subcellular location | Secreted |
| Synonyms | CCN4; WISP1c; WISP1i; WISP1tc; WNT1-inducible-signaling pathway protein 1; WISP-1; CCN family member 4; Wnt-1-induced secreted protein; WISP1 |
| Background | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is WISP1 Polyclonal Antibody, Cy5 Conjugated (bs-6321R-Cy5) validated for, and at what dilution?
bs-6321R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-6321R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WISP1 Polyclonal Antibody, Cy5 Conjugated (bs-6321R-Cy5)?
KLH conjugated synthetic peptide derived from human WISP1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6321R-Cy5)?
Cy5 on bs-6321R-Cy5: excitation / emission 625,650nm/670nm.