TRP12/TRPV4 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6425R-HRP-100uL |
| Product Name | TRP12/TRPV4 Polyclonal Antibody, HRP Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Human, Bovine |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TRPV4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59341 |
| Swiss Prot | Q9HBA0 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SMAL; VRL2; BCYM3; CMT2C; SPSMA; TRP12; VROAC; HMSN2C; OTRPC4; SSQTL1; Transient receptor potential cation channel subfamily V member 4; TrpV4; Osm-9-like TRP channel 4; Transient receptor potential protein 12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; VRL-2; Vanilloid receptor-related osmotically-activated channel; VR-OAC |
| Background | Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. |
| Application Dilution | |
| IHC-P | =1:100-500 |
| IHC-F | IHC-F=1:100-500 |
Frequently asked questions
Which applications is TRP12/TRPV4 Polyclonal Antibody, HRP Conjugated (bs-6425R-HRP) validated for, and at what dilution?
bs-6425R-HRP is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TRP12/TRPV4 Polyclonal Antibody, HRP Conjugated (bs-6425R-HRP) react with?
Tested reactivity: Human, Bovine. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken.
How should bs-6425R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRP12/TRPV4 Polyclonal Antibody, HRP Conjugated (bs-6425R-HRP)?
KLH conjugated synthetic peptide derived from human TRPV4