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ACP5 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-6434R-Cy3-100uL
Product Name ACP5 Polyclonal Antibody, Cy3 Conjugated
Applications
WB FCM
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Pig, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Tartrate-resistant acid phosphatase type 5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54
Swiss Prot P13686
Subcellular location Cytoplasm
Synonyms TRAP; SPENCDI; Tartrate-resistant acid phosphatase type 5; TR-AP; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase; ACP5; TRACP5a; TRACP5b
Background Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is ACP5 Polyclonal Antibody, Cy3 Conjugated (bs-6434R-Cy3) validated for, and at what dilution?

bs-6434R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACP5 Polyclonal Antibody, Cy3 Conjugated (bs-6434R-Cy3) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, Horse, Rabbit.

How should bs-6434R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACP5 Polyclonal Antibody, Cy3 Conjugated (bs-6434R-Cy3)?

KLH conjugated synthetic peptide derived from human Tartrate-resistant acid phosphatase type 5

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6434R-Cy3)?

Cy3 on bs-6434R-Cy3: excitation / emission 512,550nm/570,615nm.

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