ALOX15 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6505R-FITC-100uL |
| Product Name | ALOX15 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ALOX15/15 Lipoxygenase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 246 |
| Swiss Prot | P16050 |
| Subcellular location | Cytoplasm |
| Synonyms | 12-LOX; 15LOX-1; 15-LOX-1; Arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate omega-6 lipoxygenase; ALOX15; LOG15 |
| Background | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid/12-HPETE and 15-hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
Frequently asked questions
Which applications is ALOX15 Polyclonal Antibody, FITC Conjugated (bs-6505R-FITC) validated for, and at what dilution?
bs-6505R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALOX15 Polyclonal Antibody, FITC Conjugated (bs-6505R-FITC) react with?
Tested reactivity: Rat.
How should bs-6505R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALOX15 Polyclonal Antibody, FITC Conjugated (bs-6505R-FITC)?
KLH conjugated synthetic peptide derived from human ALOX15/15 Lipoxygenase 1
What are the excitation and emission wavelengths of the FITC conjugate (bs-6505R-FITC)?
FITC on bs-6505R-FITC: excitation / emission 494nm/518nm.