ABH8 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6510R-Cy5.5-100uL |
| Product Name | ABH8 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ABH8/ALKBH8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 91801 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ABH8; alkB alkylation repair homolog 8 E. coli; alkB alkylation repair homolog 8; AlkB homologue 8; ALKB8_HUMAN; ALKBH8; Alkylated DNA repair protein alkB homolog 8; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8. |
| Background | Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ABH8 Polyclonal Antibody, Cy5.5 Conjugated (BS-6510R-CY5-5) validated for, and at what dilution?
BS-6510R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ABH8 Polyclonal Antibody, Cy5.5 Conjugated (BS-6510R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Horse, Chicken, Rabbit.
How should BS-6510R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ABH8 Polyclonal Antibody, Cy5.5 Conjugated (BS-6510R-CY5-5)?
KLH conjugated synthetic peptide derived from human ABH8/ALKBH8
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6510R-CY5-5)?
Cy5.5 on BS-6510R-CY5-5: excitation / emission 675nm/694nm.