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ADORA1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-6649R-Cy3-100uL
Product Name ADORA1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Adenosine A1 Receptor
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 134
Swiss Prot P30542
Subcellular location Cell membrane
Synonyms RDC7; Adenosine receptor A1; ADORA1
Background Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response, and the inhibition of protein synthesis. The Adenosine receptors, including Adenosine A1-R, Adenosine A2A-R, Adenosine A2B-R and Adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. Adenosine A1-R mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via Adenosine A2A-R. Collagen synthesis and total protein synthesis are inhibited in certain cells by Adenosine, acting via the A2B receptors. Activation of Adenosine A3-R inhibits the induction of TNF?and blocks the endotoxin CD14 receptor signal transduction pathway.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is ADORA1 Polyclonal Antibody, Cy3 Conjugated (bs-6649R-Cy3) validated for, and at what dilution?

bs-6649R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADORA1 Polyclonal Antibody, Cy3 Conjugated (bs-6649R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-6649R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADORA1 Polyclonal Antibody, Cy3 Conjugated (bs-6649R-Cy3)?

KLH conjugated synthetic peptide derived from human Adenosine A1 Receptor

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6649R-Cy3)?

Cy3 on bs-6649R-Cy3: excitation / emission 512,550nm/570,615nm.

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