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14-3-3 zeta+delta Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-6790R-PE-100uL
Product Name 14-3-3 zeta+delta Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7534
Swiss Prot P63104
Subcellular location Cytoplasm
Synonyms HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; YWHAZ
Background Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is 14-3-3 zeta+delta Polyclonal Antibody, PE Conjugated (bs-6790R-PE) validated for, and at what dilution?

bs-6790R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 14-3-3 zeta+delta Polyclonal Antibody, PE Conjugated (bs-6790R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse, Chicken, Rabbit.

How should bs-6790R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 14-3-3 zeta+delta Polyclonal Antibody, PE Conjugated (bs-6790R-PE)?

KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta

What are the excitation and emission wavelengths of the PE conjugate (bs-6790R-PE)?

PE on bs-6790R-PE: excitation / emission 496,564nm/578nm.

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