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EBV Nuclear Antigen Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Virus
Overview
Catalog # bs-6938R-RBITC-100uL
Product Name EBV Nuclear Antigen Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Virus
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from EBV Nuclear Antigen
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms EBNA1; EBNA-1; EBV nuclear antigen 1; Epstein Barr nuclear antigen 1; Epstein Barr virus; BKRF1; Epstein-Barr nuclear antigen 1; EBV nuclear antigen 1;
Background Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is EBV Nuclear Antigen Polyclonal Antibody, RBITC Conjugated (bs-6938R-RBITC) validated for, and at what dilution?

bs-6938R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EBV Nuclear Antigen Polyclonal Antibody, RBITC Conjugated (bs-6938R-RBITC) react with?

Tested reactivity: Virus.

How should bs-6938R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EBV Nuclear Antigen Polyclonal Antibody, RBITC Conjugated (bs-6938R-RBITC)?

KLH conjugated synthetic peptide derived from EBV Nuclear Antigen

What are the excitation and emission wavelengths of the RBITC conjugate (bs-6938R-RBITC)?

RBITC on bs-6938R-RBITC: excitation / emission 540nm/625nm.

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