TOMM20 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7357r-cy55-100uL |
| Product Name | TOMM20 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TOM20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9804 |
| Swiss Prot | Q15388 |
| Subcellular location | Cytoplasm |
| Synonyms | MAS20; MOM19; TOM20; 1810060K07Rik; Gm19268; mKIAA0016; TOM20_HUMAN; TOMM20; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; KIAA0016; TOM20_MOUSE; TOM20_RAT; translocase of outer mitochondrial membrane 20; translocase of outer mitochondrial membrane 20 homolog (yeast); translocase of outer mitochondrial membrane 20 homolog type II |
| Background | Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:100-500 |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is TOMM20 Polyclonal Antibody, Cy5.5 Conjugated (BS-7357R-CY5-5) validated for, and at what dilution?
BS-7357R-CY5-5 is validated for WB (1:500-2000); IF (1:100-500); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TOMM20 Polyclonal Antibody, Cy5.5 Conjugated (BS-7357R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Horse, Chicken, Rabbit.
How should BS-7357R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TOMM20 Polyclonal Antibody, Cy5.5 Conjugated (BS-7357R-CY5-5)?
KLH conjugated synthetic peptide derived from human TOM20
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7357R-CY5-5)?
Cy5.5 on BS-7357R-CY5-5: excitation / emission 675nm/694nm.