TSPYL5 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-7703R-Cy7-100uL |
| Product Name | TSPYL5 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSPYL5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 85453 |
| Swiss Prot | Q86VY4 |
| Subcellular location | Nucleus |
| Synonyms | Testis-specific Y-encoded-like protein 5; TSPY-like protein 5; TSPYL5; KIAA1750 |
| Background | Involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53 protein levels and promotes its ubiquitination; the function is dependent on USP7 and independent on MDM2. Proposed to displace p53/TP53 from interaction with USP7. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TSPYL5 Polyclonal Antibody, Cy7 Conjugated (bs-7703R-Cy7) validated for, and at what dilution?
bs-7703R-Cy7 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSPYL5 Polyclonal Antibody, Cy7 Conjugated (bs-7703R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Horse, Rabbit.
How should bs-7703R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSPYL5 Polyclonal Antibody, Cy7 Conjugated (bs-7703R-Cy7)?
KLH conjugated synthetic peptide derived from human TSPYL5
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-7703R-Cy7)?
Cy7 on bs-7703R-Cy7: excitation / emission 743nm/767nm.