RB1CC1/FIP200 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-8165R-APC-Cy7-100uL |
| Product Name | RB1CC1/FIP200 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Chicken |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RB1CC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9821 |
| Swiss Prot | Q8TDY2 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | CC1; ATG17; FIP200; PPP1R131; RB1-inducible coiled-coil protein 1; FAK family kinase-interacting protein of 200 kDa; RB1CC1; KIAA0203; RBICC |
| Background | Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111. Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) duting S.typhimurium infection and subsequent xenophagy. Autophagy positively regulates repair of DNA damage induced by ionizing radiation and negatively regulates apoptosis. Plays an indispensible role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity). Implicated in the regulation of RB1 expression. Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Expression is, thus, a prerequisite for myogenic differentiation. Inhibits PTK2/FAK1 and PTK2B/PYK2 activity and activation of downstream signaling pathways. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RB1CC1/FIP200 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8165R-APC-Cy7) validated for, and at what dilution?
bs-8165R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RB1CC1/FIP200 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8165R-APC-Cy7) react with?
Tested reactivity: Chicken. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-8165R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RB1CC1/FIP200 Polyclonal Antibody, APC-Cy7 Conjugated (bs-8165R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human RB1CC1
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-8165R-APC-Cy7)?
APC-Cy7 on bs-8165R-APC-Cy7: excitation / emission 650nm/780nm.