UBE2D1 Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8356R-HRP-100uL |
| Product Name | UBE2D1 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBE2D1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7321 |
| Subcellular location | Cytoplasm |
| Synonyms | E217KB1; SFT; Stimulator of Fe transport; UB2D1_HUMAN; UBC 4/5; UBC4/5; UBC4/5 homolog; UBCH 5; UBCH 5A; UBCH5; UBCH5A; Ube2d1; Ubiquitin carrier protein; Ubiquitin carrier protein D1; Ubiquitin conjugating enzyme E2 17 kDa 1; Ubiquitin conjugating enzyme E2 D1; Ubiquitin conjugating enzyme E2D 1 UBC4/5 homolog yeast; Ubiquitin conjugating enzyme E2D 1; Ubiquitin protein ligase D1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E217KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1. |
| Background | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is UBE2D1 Polyclonal Antibody, HRP Conjugated (bs-8356R-HRP) validated for, and at what dilution?
bs-8356R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8356R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBE2D1 Polyclonal Antibody, HRP Conjugated (bs-8356R-HRP)?
KLH conjugated synthetic peptide derived from human UBE2D1