Luciferase Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-8539R-HRP-100uL |
| Product Name | Luciferase Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Firefly |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Firefly Luciferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7100 |
| Subcellular location | Cytoplasm |
| Synonyms | Luciferin 4-monooxygenase |
| Background | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Luciferase Polyclonal Antibody, HRP Conjugated (bs-8539R-HRP) validated for, and at what dilution?
bs-8539R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-8539R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Luciferase Polyclonal Antibody, HRP Conjugated (bs-8539R-HRP)?
KLH conjugated synthetic peptide derived from Firefly Luciferase