3-Nitrotyrosine Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-8551R-PE-Cy5.5-100uL |
| Product Name | 3-Nitrotyrosine Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated to Nitrotyrosine |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | NULL |
| Background | Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is 3-Nitrotyrosine Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8551R-PE-CY5-5) validated for, and at what dilution?
BS-8551R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does 3-Nitrotyrosine Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8551R-PE-CY5-5) react with?
Tested reactivity: Species independent.
How should BS-8551R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise 3-Nitrotyrosine Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-8551R-PE-CY5-5)?
KLH conjugated to Nitrotyrosine
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-8551R-PE-CY5-5)?
PE-Cy5.5 on BS-8551R-PE-CY5-5: excitation / emission 488nm/694nm.