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Annexin VI Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-8997R-RBITC-100uL
Product Name Annexin VI Polyclonal Antibody, RBITC Conjugated
Applications
WB IF
Specificity May have slight cross reactivity with ras-related protein Rap-2a due to 6 amino acids sequence similarity.
Reactivity Human, Mouse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Annexin VI
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 309
Subcellular location Cell membrane
Synonyms Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII; Lipocortin VI; LipocortinVI; p68; P70; Protein III; ProteinIII.
Background The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is Annexin VI Polyclonal Antibody, RBITC Conjugated (bs-8997R-RBITC) validated for, and at what dilution?

bs-8997R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Annexin VI Polyclonal Antibody, RBITC Conjugated (bs-8997R-RBITC) react with?

Tested reactivity: Human, Mouse.

How should bs-8997R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Annexin VI Polyclonal Antibody, RBITC Conjugated (bs-8997R-RBITC)?

KLH conjugated synthetic peptide derived from human Annexin VI

What are the excitation and emission wavelengths of the RBITC conjugate (bs-8997R-RBITC)?

RBITC on bs-8997R-RBITC: excitation / emission 540nm/625nm.

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