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ZSWIM3 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-9138R-Cy5-100uL
Product Name ZSWIM3 Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZSWIM3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 140831
Synonyms Zinc finger SWIM domain containing protein 3; C20orf164; Zinc finger SWIM type containing 3; ZSWIM 3; ZSWM3_HUMAN.
Background Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZSWIM3 (Zinc finger SWIM domain-containing protein 3) is a 696 amino acid protein that contains one SWIM-type zinc finger. SWIM domains are found in a variety of eukaryotic and prokaryotic proteins and are thought to be critical for certain ubiquitination reactions. The gene encoding ZSWIM3 maps to human chromosome 20, which contains nearly 63 million bases that encode over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is ZSWIM3 Polyclonal Antibody, Cy5 Conjugated (bs-9138R-Cy5) validated for, and at what dilution?

bs-9138R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZSWIM3 Polyclonal Antibody, Cy5 Conjugated (bs-9138R-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-9138R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZSWIM3 Polyclonal Antibody, Cy5 Conjugated (bs-9138R-Cy5)?

KLH conjugated synthetic peptide derived from human ZSWIM3

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9138R-Cy5)?

Cy5 on bs-9138R-Cy5: excitation / emission 625,650nm/670nm.

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