ZNRF2 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9140R-Cy7-100uL |
| Product Name | ZNRF2 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNRF2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 223082 |
| Synonyms | E3 ubiquitin-protein ligase ZNRF2; Protein Ells2; RING finger protein 202; Zinc/RING finger protein 2; Znrf2; ZNRF2_HUMAN. |
| Background | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). ZNRF2 (zinc and ring finger 2), also known as RNF202, is a 242 amino acid peripheral membrane protein that contains one RING-type zinc finger and localizes to the lysosome, as well as the endosome and the cell junction. Expressed at high levels in brain tissue, ZNRF2 is thought to function as an E3 ubiquitin-protein ligase that may be involved in the establishment and maintenance of neuronal transmission and plasticity. Upon DNA damage, ZNRF2 is subject to phosphorylation, probably by ATR or ATM. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is ZNRF2 Polyclonal Antibody, Cy7 Conjugated (bs-9140R-Cy7) validated for, and at what dilution?
bs-9140R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNRF2 Polyclonal Antibody, Cy7 Conjugated (bs-9140R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9140R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNRF2 Polyclonal Antibody, Cy7 Conjugated (bs-9140R-Cy7)?
KLH conjugated synthetic peptide derived from human ZNRF2
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-9140R-Cy7)?
Cy7 on bs-9140R-Cy7: excitation / emission 743nm/767nm.