UBOX5 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9145R-Cy3-100uL |
| Product Name | UBOX5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UBOX5/RNF37 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22888 |
| Synonyms | UIP5; UIP-5; UIP 5; RING finger protein 37; RNF37; U-box domain containing 5; U-box domain-containing protein 5; UBCE7IP5; ubiquitin conjugating enzyme 7 interacting protein 5; UBOX5; RNF37_HUMAN. |
| Background | The U-box domain is a modified RING finger motif that has been implicated in the ubiquitin/proteasome system. The ubiquitin-conjugating enzyme 7-interacting protein 5 (UIP5), also designated U-box domain-containing protein 5 or RING finger protein 37, contains 1 RING-type zinc finger and 1 U-box domain. UIP5 has been shown to interact with UBCH7, an enzyme that mediates selective degradation of abnormal proteins. The gene encoding UIP5 maps to chromosome 20, which houses over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. Additionally, chromosome 20 contains a region with numerous genes which are thought important for seminal production and may be potential targets for male contraception. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is UBOX5 Polyclonal Antibody, Cy3 Conjugated (bs-9145R-Cy3) validated for, and at what dilution?
bs-9145R-Cy3 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UBOX5 Polyclonal Antibody, Cy3 Conjugated (bs-9145R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-9145R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UBOX5 Polyclonal Antibody, Cy3 Conjugated (bs-9145R-Cy3)?
KLH conjugated synthetic peptide derived from human UBOX5/RNF37
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9145R-Cy3)?
Cy3 on bs-9145R-Cy3: excitation / emission 512,550nm/570,615nm.