Transcription factor 25 Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9604R-Cy5.5-100uL |
| Product Name | Transcription factor 25 Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Transcription factor 25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22980 |
| Synonyms | FKSG26; hKIAA1049; NULP1; PRO2620; Hulp1; KIAA1049; Nuclear localized protein 1; Transcription factor 25 basic helix loop helix; TCF25_HUMAN. |
| Background | Transcription factor 25 acts as a transcriptional repressor. It has been shown to repress transcription of SRF in vitro and hence may play a role in heart development.Transcription factor 25, is a 676 amino acid protein that plays a role in cell death. A member of the TCF25 family, Nulp1 utilizes its C-terminus to mediate transcriptional repression of SRF in vitro, and interacts with XIAP. Nulp1 localizes primarily to the nucleus but is also found in cytosol. Widely expressed, Nulp1 is found at high levels in embryonic brain and adult heart. The gene encoding Nulp1 maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Transcription factor 25 Antibody, Cy5.5 Conjugated (BS-9604R-CY5-5) validated for, and at what dilution?
BS-9604R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Transcription factor 25 Antibody, Cy5.5 Conjugated (BS-9604R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9604R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Transcription factor 25 Antibody, Cy5.5 Conjugated (BS-9604R-CY5-5)?
KLH conjugated synthetic peptide derived from human Transcription factor 25
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9604R-CY5-5)?
Cy5.5 on BS-9604R-CY5-5: excitation / emission 675nm/694nm.