ZWILCH Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9732R-Biotin-100uL |
| Product Name | ZWILCH Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZWILCH |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 55055 |
| Swiss Prot | Q9H900 |
| Synonyms | hZwilch; KNTC1AP; MGC111034; Protein zwilch homolog; ZWILC_HUMAN; zwilch;y ZWILCH protein; Zwilch, kinetochore associated, homolog. |
| Background | Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is ZWILCH Polyclonal Antibody, Biotin Conjugated (bs-9732R-Biotin) validated for, and at what dilution?
bs-9732R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZWILCH Polyclonal Antibody, Biotin Conjugated (bs-9732R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9732R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZWILCH Polyclonal Antibody, Biotin Conjugated (bs-9732R-Biotin)?
KLH conjugated synthetic peptide derived from human ZWILCH