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Staufen Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-9877R-RBITC-100uL
Product Name Staufen Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Horse, Chicken
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Staufen
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6780
Swiss Prot O95793
Subcellular location Cytoplasm
Synonyms STAU; PPP1R150; Double-stranded RNA-binding protein Staufen homolog 1; STAU1
Background Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Staufen Polyclonal Antibody, RBITC Conjugated (bs-9877R-RBITC) validated for, and at what dilution?

bs-9877R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Staufen Polyclonal Antibody, RBITC Conjugated (bs-9877R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.

How should bs-9877R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Staufen Polyclonal Antibody, RBITC Conjugated (bs-9877R-RBITC)?

KLH conjugated synthetic peptide derived from human Staufen

What are the excitation and emission wavelengths of the RBITC conjugate (bs-9877R-RBITC)?

RBITC on bs-9877R-RBITC: excitation / emission 540nm/625nm.

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