TNF alpha Monoclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-0387m-apc-cy55-100ul |
| Product Name | TNF alpha Monoclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Specificity | This antibody is produced using full length recombinant TNF-alpha protein. |
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Mouse |
| Source | This antibody is produced using full length recombinant human TNF-alpha. |
| Immunogen Range | 77-233/233 |
| Clonality | Monoclonal |
| Clone # | 1F6 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7124 |
| Swiss Prot | P01375 |
| Subcellular location | Secreted, Membrane bound |
| Synonyms | DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF |
| Background | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is TNF alpha Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-0387M-APC-CY5-5) validated for, and at what dilution?
BSM-0387M-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TNF alpha Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-0387M-APC-CY5-5) react with?
Tested reactivity: Human.
How should BSM-0387M-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TNF alpha Monoclonal Antibody, APC-Cy5.5 Conjugated (BSM-0387M-APC-CY5-5)?
This antibody is produced using full length recombinant human TNF-alpha. Immunogen range: 77-233/233.
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-0387M-APC-CY5-5)?
APC-Cy5.5 on BSM-0387M-APC-CY5-5: excitation / emission 650nm/694nm.