Human Serum albumin Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-0945m-fitc-100uL |
| Product Name | Human Serum albumin Monoclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Pig |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | human Serum albumin |
| Clonality | Monoclonal |
| Clone # | 3F4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 213 |
| Swiss Prot | P02768 |
| Synonyms | ALB; ALBU_HUMAN; Albumin 32 AA; Albumin AA 34; Analbuminemia; Bisalbuminemia; Cell growth inhibiting protein 42; DKFZp779N1935; Dysalbuminemic hyperthyroxinemia; Growth inhibiting protein 20; HSA; Hyperthyroxinemia dysalbuminemic; PRO0883; PRO0903; PRO1341; PRO2044; PRO2619; Serum albumin. |
| Background | Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Human Serum albumin Monoclonal Antibody, FITC Conjugated (bsm-0945M-FITC) validated for, and at what dilution?
bsm-0945M-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Human Serum albumin Monoclonal Antibody, FITC Conjugated (bsm-0945M-FITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Pig.
How should bsm-0945M-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Human Serum albumin Monoclonal Antibody, FITC Conjugated (bsm-0945M-FITC)?
human Serum albumin
What are the excitation and emission wavelengths of the FITC conjugate (bsm-0945M-FITC)?
FITC on bsm-0945M-FITC: excitation / emission 494nm/518nm.