mouse CD16/32 Monoclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-30155a-fitc-100T |
| Product Name | mouse CD16/32 Monoclonal Antibody, FITC Conjugated |
| Applications |
FLOW-CYT
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | FITC |
| Host | Rat |
| Clonality | Monoclonal |
| Clone # | 6G10 |
| Isotype | Rat IgG2a, k |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 14131 |
| Swiss Prot | P08508 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | CD16; CD16-II; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; FcGRIIIA; IGFR3; IMD20; CD16-2; Fcgr4; FCG3A_HUMAN; FCGR3A; IgG Fc receptor III-A; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII | Fc-gamma RIIIa | FcRIII | FcRIIIa | FcgammaRIIIA); IgG Fc receptor III-2; FCGR3_MOUSE; IgG Fc receptor III; Fc-gamma RIII (FcRIII); FCGR3_RAT; Fc gamma receptor IIIa; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG receptor IIIa; FcγR III; |
| Background | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] |
| Application Dilution | |
| Flow-Cyt | 1:50-100 |
Frequently asked questions
Which applications is mouse CD16/32 Monoclonal Antibody, FITC Conjugated (bsm-30155A-FITC) validated for, and at what dilution?
bsm-30155A-FITC is validated for Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does mouse CD16/32 Monoclonal Antibody, FITC Conjugated (bsm-30155A-FITC) react with?
Tested reactivity: Mouse.
How should bsm-30155A-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the FITC conjugate (bsm-30155A-FITC)?
FITC on bsm-30155A-FITC: excitation / emission 494nm/518nm.