Trx-Tag Monoclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33018m-bf647-100uL |
| Product Name | Trx-Tag Monoclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Pig, Species independent |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Mouse |
| Source | Recombinant human Thioredoxin |
| Clonality | Monoclonal |
| Clone # | 7G10 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7295 |
| Swiss Prot | P10599 |
| Subcellular location | Cytoplasm, Nucleus, Secreted |
| Synonyms | Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903; Trx-Tag. |
| Background | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Trx-Tag Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33018M-BF647) validated for, and at what dilution?
bsm-33018M-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Trx-Tag Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33018M-BF647) react with?
Tested reactivity: Human, Pig, Species independent.
How should bsm-33018M-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Trx-Tag Monoclonal Antibody, AbBy Fluor-647 Conjugated (bsm-33018M-BF647)?
Recombinant human Thioredoxin
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-33018M-BF647)?
AbBy Fluor-647 on bsm-33018M-BF647: excitation / emission 650nm/665nm.