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beta-Actin Monoclonal Antibody, APC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Pig
Overview
Catalog # bsm-33036m-apc-100uL
Product Name beta-Actin Monoclonal Antibody, APC Conjugated
Applications
WB IF
Specificity The immunogen of this antibody has sequence similarity to actin cytoplasmic 2 and beta-actin-like protein 2, and may bind to these targets.
Reactivity Human, Mouse, Rat, Bovine, Pig
Specifications
Conjugation APC
Host Mouse
Source KLH conjugated synthetic peptide derived from human beta-Actin
Clonality Monoclonal
Clone # 1A2
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 60
Swiss Prot P60709
Synonyms Actin, cytoplasmic 1; ACTB
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Application Dilution
WB 1:300-5000
IF 1:300-5000

Frequently asked questions

Which applications is beta-Actin Monoclonal Antibody, APC Conjugated (bsm-33036M-APC) validated for, and at what dilution?

bsm-33036M-APC is validated for WB (1:300-5000); IF (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does beta-Actin Monoclonal Antibody, APC Conjugated (bsm-33036M-APC) react with?

Tested reactivity: Human, Mouse, Rat, Bovine, Pig.

How should bsm-33036M-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise beta-Actin Monoclonal Antibody, APC Conjugated (bsm-33036M-APC)?

KLH conjugated synthetic peptide derived from human beta-Actin

What are the excitation and emission wavelengths of the APC conjugate (bsm-33036M-APC)?

APC on bsm-33036M-APC: excitation / emission 650nm/660nm.

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