Histone H3(di methyl K27) Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33107m-rbitc-100uL |
| Product Name | Histone H3(di methyl K27) Monoclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Histone H3(di methyl K27) |
| Clonality | Monoclonal |
| Clone # | 5C11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4 ; Histone H3.1t ; HIST3H3; HGNC:4778; H31T_HUMAN |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Application Dilution | |
| WB | 1ug/Test |
| IF(ICC) | 1:200-1000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is Histone H3(di methyl K27) Monoclonal Antibody, RBITC Conjugated (bsm-33107M-RBITC) validated for, and at what dilution?
bsm-33107M-RBITC is validated for WB (1ug/Test); IF(ICC) (1:200-1000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3(di methyl K27) Monoclonal Antibody, RBITC Conjugated (bsm-33107M-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33107M-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3(di methyl K27) Monoclonal Antibody, RBITC Conjugated (bsm-33107M-RBITC)?
KLH conjugated synthetic peptide derived from human Histone H3(di methyl K27)
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-33107M-RBITC)?
RBITC on bsm-33107M-RBITC: excitation / emission 570nm/595nm.