mCherry (9F11) Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33131m-cy55-100ul |
| Product Name | mCherry (9F11) Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Mouse |
| Source | Recombinant Protein mCherry |
| Clonality | Monoclonal |
| Clone # | 9F11 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | mCherry; mCherry Tag; monomeric cherry red fluorescent protein. |
| Background | mCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively. It is resistant to photobleaching and is stable. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is mCherry (9F11) Monoclonal Antibody, Cy5.5 Conjugated (BSM-33131M-CY5-5) validated for, and at what dilution?
BSM-33131M-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does mCherry (9F11) Monoclonal Antibody, Cy5.5 Conjugated (BSM-33131M-CY5-5) react with?
Tested reactivity: Species independent.
How should BSM-33131M-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise mCherry (9F11) Monoclonal Antibody, Cy5.5 Conjugated (BSM-33131M-CY5-5)?
Recombinant Protein mCherry
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-33131M-CY5-5)?
Cy5.5 on BSM-33131M-CY5-5: excitation / emission 675nm/694nm.