TAP-Tag Monoclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33133M-HRP-100uL |
| Product Name | TAP-Tag Monoclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | HRP |
| Host | Mouse |
| Source | Recombinant TAP-Tag |
| Clonality | Monoclonal |
| Clone # | 4F7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | TAP Tag; TAPTag; Tandem affinity purification |
| Background | Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is TAP-Tag Monoclonal Antibody, HRP Conjugated (bsm-33133M-HRP) validated for, and at what dilution?
bsm-33133M-HRP is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TAP-Tag Monoclonal Antibody, HRP Conjugated (bsm-33133M-HRP) react with?
Tested reactivity: Species independent.
How should bsm-33133M-HRP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TAP-Tag Monoclonal Antibody, HRP Conjugated (bsm-33133M-HRP)?
Recombinant TAP-Tag