beta-Actin Monoclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33139m-pe-cy5-100ul |
| Product Name | beta-Actin Monoclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human beta-Actin |
| Clonality | Monoclonal |
| Clone # | 1A5 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 60 |
| Swiss Prot | P60709 |
| Synonyms | Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN. |
| Background | Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is beta-Actin Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33139M-PE-Cy5) validated for, and at what dilution?
bsm-33139M-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta-Actin Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33139M-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33139M-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta-Actin Monoclonal Antibody, PE-Cy5 Conjugated (bsm-33139M-PE-Cy5)?
KLH conjugated synthetic peptide derived from human beta-Actin
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-33139M-PE-Cy5)?
PE-Cy5 on bsm-33139M-PE-Cy5: excitation / emission 488nm/670nm.