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Vimentin Monoclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
Overview
Catalog # bsm-33170m-bf750-100uL
Product Name Vimentin Monoclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF FLOW-CYT
Specificity KO-Validated
Reactivity Human
Specifications
Conjugation AbBy Fluor-750
Host Mouse
Source Recombinant human Vimentin Protein
Clonality Monoclonal
Clone # 3B7
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7431
Swiss Prot P08670
Synonyms Vimentin; VIM
Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 Publication Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Application Dilution
WB 1:500-2000
IF 1:50-200
Flow-Cyt 1ug/Test

Frequently asked questions

Which applications is Vimentin Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33170M-BF750) validated for, and at what dilution?

bsm-33170M-BF750 is validated for WB (1:500-2000); IF (1:50-200); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Vimentin Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33170M-BF750) react with?

Tested reactivity: Human.

How should bsm-33170M-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Vimentin Monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33170M-BF750)?

Recombinant human Vimentin Protein

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-33170M-BF750)?

AbBy Fluor-750 on bsm-33170M-BF750: excitation / emission 749nm/775nm.