alpha smooth muscle Actin Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33188m-cy3-100uL |
| Product Name | alpha smooth muscle Actin Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin |
| Clonality | Monoclonal |
| Clone # | 8B2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59 |
| Swiss Prot | P62736 |
| Synonyms | alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5_-Smooth Muscle Actin; _ Smooth Muscle Actin; |
| Background | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is alpha smooth muscle Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33188M-Cy3) validated for, and at what dilution?
bsm-33188M-Cy3 is validated for WB (1:300-5000); IF (1:50-200); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha smooth muscle Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33188M-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33188M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha smooth muscle Actin Monoclonal Antibody, Cy3 Conjugated (bsm-33188M-Cy3)?
KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-33188M-Cy3)?
Cy3 on bsm-33188M-Cy3: excitation / emission 512,550nm/570,615nm.