Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33215m-percp-cy55-100ul |
| Product Name | Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide of Nano-Tag (9) |
| Clonality | Monoclonal |
| Clone # | 6B7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Nano-Tag (9); Nano-Tag9; Nano-Tag; Nano Tag (9); |
| Background | Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The Nano-tag is a new streptavidin-binding peptide for both the purification and the detection of Nano-tagged proteins. This peptide possesses nanomolar-affinity for streptavidin and therefore is termed Nano-tag. The nano-tags have two types, Nano-tag15 (MDVEAWLGARVPLVET) and Nano-tag9 (MDVEAWLGAR), which bind to streptavidin with dissociation constants of 4 nM and 17 nM, respectively. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-33215M-PERCP-CY5-5) validated for, and at what dilution?
BSM-33215M-PERCP-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-33215M-PERCP-CY5-5) react with?
Tested reactivity: Species independent.
How should BSM-33215M-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nano-Tag (9) (6B7) Monoclonal Antibody, PerCP-Cy5.5 Conjugated (BSM-33215M-PERCP-CY5-5)?
KLH conjugated synthetic peptide of Nano-Tag (9)
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-33215M-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-33215M-PERCP-CY5-5: excitation / emission 482nm/694nm.