TBP Monoclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33227M-BF488-100uL |
| Product Name | TBP Monoclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Mouse |
| Source | Recombinant human TBP protein |
| Clonality | Monoclonal |
| Clone # | 6G8 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | P20226 |
| Swiss Prot | 6908 |
| Synonyms | GTF2D; TFIID; GTF2D1; MGC117320; MGC126054; MGC126055; SCA 17; SCA17; TATA binding factor; TATA box binding protein; TATA box factor; TATA sequence binding protein; TATA sequence-binding protein; TATA binding protein TBP; TATA-binding factor; TATA-box factor; TATA-box-binding protein; TBP; TBP_HUMAN; TF2D; TFIID; Transcription initiation factor TFIID TBP subunit. |
| Background | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is TBP Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-33227M-BF488) validated for, and at what dilution?
bsm-33227M-BF488 is validated for WB (1:300-5000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TBP Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-33227M-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-33227M-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TBP Monoclonal Antibody, AbBy Fluor-488 Conjugated (bsm-33227M-BF488)?
Recombinant human TBP protein
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-33227M-BF488)?
AbBy Fluor-488 on bsm-33227M-BF488: excitation / emission 499nm/519nm.