Bcl-2 monoclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-33413M-BF750-100uL |
| Product Name | Bcl-2 monoclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Bcl-2 |
| Immunogen Range | 51-150/239 |
| Clonality | Monoclonal |
| Clone # | 4H1 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 596 |
| Swiss Prot | P10415 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN. |
| Background | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8). |
| Application Dilution | |
| WB | =1:500-2000 |
| Flow-Cyt | Flow-Cyt=1:100 |
Frequently asked questions
Which applications is Bcl-2 monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33413M-BF750) validated for, and at what dilution?
bsm-33413M-BF750 is validated for WB (WB=1:500-2000); Flow-Cyt (Flow-Cyt=1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33413M-BF750) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig.
How should bsm-33413M-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 monoclonal Antibody, AbBy Fluor-750 Conjugated (bsm-33413M-BF750)?
KLH conjugated synthetic peptide derived from human Bcl-2 Immunogen range: 51-150/239.
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-33413M-BF750)?
AbBy Fluor-750 on bsm-33413M-BF750: excitation / emission 749nm/775nm.