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HIF-1 Alpha Monoclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-33428m-bf405-100uL
Product Name HIF-1 Alpha Monoclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Mouse
Source Recombinant human HIF-1 Alpha Protein
Clonality Monoclonal
Clone # 3G9
Isotype IgG1
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3091
Swiss Prot Q16665
Synonyms ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1a; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8.
Application Dilution
WB 1:1000-5000
IF(ICC) IFICC1:100-500

Frequently asked questions

Which applications is HIF-1 Alpha Monoclonal Antibody, AbBy Fluor-405 Conjugated (bsm-33428M-BF405) validated for, and at what dilution?

bsm-33428M-BF405 is validated for WB(WB) (1:1000-5000); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HIF-1 Alpha Monoclonal Antibody, AbBy Fluor-405 Conjugated (bsm-33428M-BF405) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-33428M-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HIF-1 Alpha Monoclonal Antibody, AbBy Fluor-405 Conjugated (bsm-33428M-BF405)?

Recombinant human HIF-1 Alpha Protein

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-33428M-BF405)?

AbBy Fluor-405 on bsm-33428M-BF405: excitation / emission 401nm/421nm.

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