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alpha Estradiol Monoclonal Antibody, APC Conjugated

Applications

  • IF(IHC-P)

Reactivity

  • Others
Overview
Catalog # bsm-4533M-APC-100uL
Product Name alpha Estradiol Monoclonal Antibody, APC Conjugated
Applications
IF(IHC-P)
Reactivity Others
Specifications
Conjugation APC
Host Mouse
Source KLH conjugated alpha Estradiol
Clonality Monoclonal
Clone # 2G2
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms 1,3,510-Estratriene-3,17alpha-diol; estrogen; 3,17 alpha-Dihydroxy-1,3,510-estratriene; Epiestradiol; alpha Estradiol; 17 alpha Estradiol; Ethynyl Estradiol; Oestradiol; Estradial; Ovocylin; biologically active estrogen in human breast cancer cells.
Background Estradiol (known as alpha Estradiol or 17 alpha Estradiol) is a biologically active estrogen in human breast cancer cells in tissue culture.17-estradiol and its selective receptor, ER-X, are not part of a classical hormone/receptor endocrine system but of a system with important autocrine/paracrine functions in the developing and adult brain. 17-Estradiol may have enormous implications for hormone replacement strategies at the menopause and in the treatment of such neurodegenerative disorders as Alzheimer?s disease and ischemic stroke.
Application Dilution
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is alpha Estradiol Monoclonal Antibody, APC Conjugated (bsm-4533M-APC) validated for, and at what dilution?

bsm-4533M-APC is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bsm-4533M-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise alpha Estradiol Monoclonal Antibody, APC Conjugated (bsm-4533M-APC)?

KLH conjugated alpha Estradiol

What are the excitation and emission wavelengths of the APC conjugate (bsm-4533M-APC)?

APC on bsm-4533M-APC: excitation / emission 650nm/660nm.

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