alpha Estradiol Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-4533M-Cy3-100uL |
| Product Name | alpha Estradiol Monoclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Others |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | KLH conjugated alpha Estradiol |
| Clonality | Monoclonal |
| Clone # | 2G2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | 1,3,510-Estratriene-3,17alpha-diol; estrogen; 3,17 alpha-Dihydroxy-1,3,510-estratriene; Epiestradiol; alpha Estradiol; 17 alpha Estradiol; Ethynyl Estradiol; Oestradiol; Estradial; Ovocylin; biologically active estrogen in human breast cancer cells. |
| Background | Estradiol (known as alpha Estradiol or 17 alpha Estradiol) is a biologically active estrogen in human breast cancer cells in tissue culture.17-estradiol and its selective receptor, ER-X, are not part of a classical hormone/receptor endocrine system but of a system with important autocrine/paracrine functions in the developing and adult brain. 17-Estradiol may have enormous implications for hormone replacement strategies at the menopause and in the treatment of such neurodegenerative disorders as Alzheimer?s disease and ischemic stroke. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is alpha Estradiol Monoclonal Antibody, Cy3 Conjugated (bsm-4533M-Cy3) validated for, and at what dilution?
bsm-4533M-Cy3 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-4533M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha Estradiol Monoclonal Antibody, Cy3 Conjugated (bsm-4533M-Cy3)?
KLH conjugated alpha Estradiol
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-4533M-Cy3)?
Cy3 on bsm-4533M-Cy3: excitation / emission 512,550nm/570,615nm.