Human Ig Kappa light chain Monoclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-51106m-biotin-100uL |
| Product Name | Human Ig Kappa light chain Monoclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Mouse |
| Source | Recombinant human Kappa light chain |
| Clonality | Monoclonal |
| Clone # | 6H8 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3514 |
| Swiss Prot | P01834 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | HCAK1; IGKCD; Km; IGKC_HUMAN; IGKC; Ig kappa chain C region; Ig kappa chain C region AG; Ig kappa chain C region CUM; Ig kappa chain C region EU; Ig kappa chain C region OU; Ig kappa chain C region ROY; Ig kappa chain C region TI; Igk-C; IGKC_MOUSE; Ig kappa chain C region MOPC 21; |
| Background | Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two types of light chains designated as kappa and lambda (1). Light chain types are based on differences in the amino acid sequence in the constant region of the light chain. If a cell is unsuccessful in rearranging both of its kappa light chain genes, it then attempts to make a lambda light chain. If a cell successfully rearranges a lambda light chain gene, it will be a B cell that makes an immunoglobulin with a lambda light chain (2). |
| Application Dilution | |
| WB | 1:1000-5000 |
| ELISA | 1:5000-10000 |
Frequently asked questions
Which applications is Human Ig Kappa light chain Monoclonal Antibody, Biotin Conjugated (bsm-51106M-Biotin) validated for, and at what dilution?
bsm-51106M-Biotin is validated for WB (1:1000-5000); ELISA (1:5000-10000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Human Ig Kappa light chain Monoclonal Antibody, Biotin Conjugated (bsm-51106M-Biotin) react with?
Tested reactivity: Human.
How should bsm-51106M-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Human Ig Kappa light chain Monoclonal Antibody, Biotin Conjugated (bsm-51106M-Biotin)?
Recombinant human Kappa light chain